abstract：:The age-related changes in the distribution of enkephalin-immunoreactive (ENK-IR) nerve fibers in relation to the principal neurons were studied in 23 human sympathetic ganglia from patients aged between 22 and 98 years. There were no age-related changes in the diameter of nerve cells and the packing density of the ne...

journal_title：Mechanisms of ageing and development

authors： Järvi R,Helen P,Pelto-Huikko M,Rapoport SI,Hervonen A

更新日期：1988-08-01 00:00:00

abstract：:A comparison was made of the resting membrane potential (MP) as well as the MP responses to K+, ACTH and gamma-MSH of superfused adrenocortical, zona fasciculata cells from young (Y) and old (O) male rats. The resting MP of these cells did not vary with age. However, the MP responses to altered extracellular [K+] vari...

journal_title：Mechanisms of ageing and development

authors： Lymangrover JR,Valego NK,Shilling RD

更新日期：1988-06-01 00:00:00

abstract：:The effects of age and nutritional conditions on accumulation of autofluorescent granules in various organs and tissues of male Sprague-Dawley rats were compared morphometrically. The relative intensity of the specific fluorescence of these autofluorescent granules was similar in all tissues and cells examined. In alm...

journal_title：Mechanisms of ageing and development

authors： Sato T,Tokoro Y,Tauchi H,Kohtani K,Mizuno T,Shimasaki H,Ueta N

更新日期：1988-06-01 00:00:00

abstract：:Alteration of T cell surface marker expression with a decrease of CD3 positive cells relative to the number of CD4 and CD8 positive cells, diminished in vitro proliferative response to mitogenic stimuli like PHA and antibodies to the CD3/Ti complex, and increase in serum IL-2 receptor levels, are among the changes in ...

journal_title：Mechanisms of ageing and development

authors： Hallgren HM,Bergh N,Rodysill KJ,O'Leary JJ

更新日期：1988-05-01 00:00:00

abstract：:The activities of the low-Km and high-Km forms of cyclic AMP phosphodiesterase (PDE) were determined in extracts prepared from epididymis and prostate taken from adult (10-12 months) and from aged (32-35 months) rats. The maximum velocity V of low-Km PDE in the epididymis of normal adult rats (approx. 300 pmoles/min p...

journal_title：Mechanisms of ageing and development

authors： Razzaboni B,Terner C

更新日期：1988-04-01 00:00:00

abstract：:Survival characteristics of seven different populations of the mollusc Lymnaea stagnalis were studied under constant culture conditions. On the basis of these characteristics four populations were considered as healthy and three as infected. In the healthy populations senescence started at an age of about 200 days, 50...

journal_title：Mechanisms of ageing and development

authors： Janse C,Slob W,Popelier CM,Vogelaar JW

更新日期：1988-03-01 00:00:00

abstract：:Non-proliferative and proliferative myeloid, and lymphoid and erythroid bone marrow cells were studied in aging female C3H mice. A chronobiological approach was used and mice aged 16, 21 and 26 months were examined vs. 3 month-old mice every 3 h during the 24-h period in three different experiments. Significant circad...

journal_title：Mechanisms of ageing and development

authors： Sletvold O,Laerum OD,Riise T

更新日期：1988-01-01 00:00:00

abstract：:The plasma disappearance of endotoxin and endotoxin-induced hepatic injury were studied in two rat models: the aging rat and the subacutely hypervitaminotic A rat. The choice of these models was based on their respective association with a decreased or increased Kupffer cell endocytic activity. The half-life of endoto...

journal_title：Mechanisms of ageing and development

authors： Hendriks HF,Horan MA,Durham SK,Earnest DL,Brouwer A,Hollander CF,Knook DL

更新日期：1987-12-01 00:00:00

abstract：:This investigation represents the largest study so far published on human DNA damage and aging. The subject of this investigation is damage, determined as DNA alterations which give rise to complete molecular breaks in the course of treatment of purified DNA solutions with single-strand-specific nucleases. The DNA is ...

journal_title：Mechanisms of ageing and development

authors： Zahn RK,Reinmüller J,Beyer R,Pondeljak V

更新日期：1987-11-01 00:00:00

abstract：:The B cell responsiveness mediated by T cells has been evaluated in 50 aged donors by using a Pokeweed mitogen (PWM)-driven differentiation system. Peripheral blood mononuclear cells (PBMC) from aged donors exhibited a lower plaque-forming cell (PFC) generation in comparison with young donors. Similar results were obt...

journal_title：Mechanisms of ageing and development

authors： Antonaci S,Jirillo E,Gallitelli M,Garofalo AR,Bonomo L

更新日期：1987-11-01 00:00:00

abstract：:The ability of resting and dividing rabbit articular chondrocytes to repair low doses of ultraviolet (UV) damage was measured through removal of UV endonuclease-sensitive sites (ESS, pyrimidines dimers) as measured by alkaline elution. The repair of damage was significantly (P less than 0.001) greater in dividing than...

journal_title：Mechanisms of ageing and development

authors： Lipman JM,Setlow RB

更新日期：1987-09-30 00:00:00

abstract：:Putrescine, spermidine, spermine and two unknowns designated as A and B were detected in first seedling leaves of barley (Hordeum vulgare L. var. Wolfe). The levels of these polyamines in first seedling leaves from 4-day-old barley plants grown in darkness or in light were comparable and did not change significantly a...

journal_title：Mechanisms of ageing and development

authors： Srivastava BI

更新日期：1987-09-14 00:00:00

abstract：:Hepatic growth and protein synthesis in vivo was studied with age in ad libitum-fed and dietary restricted rats in which the mean and maximum lifespan was significantly extended. Livers from underfed rats showed significantly lower DNA, RNA and protein contents, and total protein synthesis. The fractional rate of synt...

journal_title：Mechanisms of ageing and development

authors： Merry BJ,Holehan AM,Lewis SE,Goldspink DF

更新日期：1987-07-01 00:00:00

abstract：:The activity of low Km cAMP-phosphodiesterase (PDE) was determined in extracts of prostate and heart of adult (10-12 months old) and aged (32-35 months old) Sprague-Dawley rats; the enzyme's response to endogenous inhibitors extracted from the two organs was analyzed by kinetic studies. Different mechanisms of inhibit...

journal_title：Mechanisms of ageing and development

authors： Moses MA,Szabo EI,Terner C

更新日期：1987-07-01 00:00:00

abstract：:In an interspecies comparison of seven primate species, the expression of the erbB proto oncogene was found to be higher in fibroblasts derived from three relatively long-lived species, the human, gorilla, and chimpanzee than in cells from the orangutan, pygmy chimpanzee, squirrel monkey, or red-bellied tamarin. No si...

journal_title：Mechanisms of ageing and development

authors： Nakamura KD,Hart RW

更新日期：1987-07-01 00:00:00

abstract：:Age-related changes in intestinal glucose absorption were studied using everted intestinal sacs and brush border membrane vesicles prepared from male F344 rats. Glucose uptake by everted intestinal sacs was greatest in young (2-3-month-old) as compared with adult (12-14-month-old) and old (24-month-old) rats. The grea...

journal_title：Mechanisms of ageing and development

authors： Doubek WG,Armbrecht HJ

更新日期：1987-06-01 00:00:00

abstract：:As measured by X-ray microanalysis of frozen-dried cryosections, physiological concentrations of phosphorus, Na+, K+ and Mg2+ show a biphasic age dependence in some compartments of rat hepatocytes, especially in the regions of condensed chromatin associated with the actively transcribed genes. This could be consistent...

journal_title：Mechanisms of ageing and development

authors： von Zglinicki T,Bimmler M

更新日期：1987-04-01 00:00:00

abstract：:The kinetics of inactivation of plasma albumin was studied in young (3-4 months) and old (25-28 months) Sprague-Dawley female rats. Conscious, free-moving animals carrying indwelling atrial and carotid cannulas received a single injection of [125I]-albumin (rat) via the carotid cannula. Sequential blood samples were r...

journal_title：Mechanisms of ageing and development

authors： Goya RG,Sosa YE,Chang J,Meites J

更新日期：1986-11-28 00:00:00

abstract：:The effect of age on the capacity of tyramine to promote the release of norepinephrine (NE) at the cardiac adrenergic neuroeffector junction was investigated in isolated hearts of rats, 6, 12 and 24 months of age. Hearts were perfused by the method of Langendorff and tyramine was administered in increasing doses as a ...

journal_title：Mechanisms of ageing and development

authors： Kreider MS,Goldberg PB,Roberts J

更新日期：1986-11-14 00:00:00

abstract：:Components of the calmodulin system (i.e., calmodulin levels and activities of the following calmodulin-dependent enzymes: Ca2+ + Mg2+-ATPase, adenylate and guanylate cyclases, cyclic AMP and cyclic GMP phosphodiesterases, and Ca2+-dependent protein kinase were studied in the following brain regions from immature (25-...

journal_title：Mechanisms of ageing and development

authors： Hoskins B,Ho IK

更新日期：1986-10-01 00:00:00

abstract：:Keratinocytes and fibroblasts were obtained from upper arm biopsies of young (22-27 years) and old (60-82 years) adult donors. Keratinocytes were grown in serum-free medium containing variable quantities of either epidermal growth factor (EGF) or a bovine hypothalamic extract known to contain keratinocyte growth facto...

journal_title：Mechanisms of ageing and development

authors： Stanulis-Praeger BM,Gilchrest BA

更新日期：1986-07-01 00:00:00

abstract：:Steroid-receptor complexes (SRCs) (glucocorticoids, estrogens and progestins) are isolated from rat liver and purified 1500-2000-fold. Both the initial cytosol complexes and those purified 2000-fold were characterized by gel filtration on Sephadex G-100 and DEAE-cellulose chromatography. The purified rat liver SRCs we...

journal_title：Mechanisms of ageing and development

authors： Konoplya EF,Luksha GL,Savateev SK,Naumov AD

更新日期：1986-07-01 00:00:00

abstract：:The basic genetic defect in the Hutchinson-Gilford Progeria Syndrome (progeria), a premature aging syndrome, is unknown. To investigate possible defects in hyaluronic acid (HA) metabolism in this disease, the urinary excretion of HA was studied. Urine specimens from 11 patients with this disorder were examined for HA ...

journal_title：Mechanisms of ageing and development

authors： Zebrower M,Kieras FJ,Brown WT

更新日期：1986-06-01 00:00:00

abstract：:Normal diploid Syrian hamster dermal mesenchymal cell strains, regardless of the age of the tissue of origin, exhibit in vitro cellular senescence. The frequency of spontaneous escape from senescence and conversion to a permanent cell line is less than 5% among replicate flasks. The overall pattern of senescence of ce...

journal_title：Mechanisms of ageing and development

authors： Bruce SA,Deamond SF,Ts'o PO

更新日期：1986-04-01 00:00:00

abstract：:A subset of 43 [35S]methionine labeled mitochondrial proteins, identified on two dimensional electrophoresis gels of whole body extracts of adult Drosophila, were compared between two age groups. These flies, which except for their spermatocytes consist entirely of postmitotic cells, were 6 days old and 38 days old, w...

journal_title：Mechanisms of ageing and development

authors： Fleming JE,Melnikoff PS,Latter GI,Chandra D,Bensch KG

更新日期：1986-03-01 00:00:00

abstract：:Filopodia in log and stationary phase populations of human fetal lung fibroblasts (WI-38) at low and high population doubling levels (PDLs) and of SV40 transformed WI-38 cells (VA13A), were observed and counted under different conditions of in vitro growth by scanning electron microscopy. Cells from old non-vigorously...

journal_title：Mechanisms of ageing and development

authors： Stanulis-Praeger BM

更新日期：1986-02-01 00:00:00

abstract：:Pancreatic islet B cells from Sprague-Dawley and Fisher 344 rats aged 3-27 months were separated from A and D cells by centrifugation over a linear percoll density gradient, and incubated in vitro with various concentrations of glucose and somatostatin. Elevation of glucose concentration in the incubation medium from ...

journal_title：Mechanisms of ageing and development

authors： Magal E,Chaudhuri M,Adelman RC

更新日期：1986-01-01 00:00:00

abstract：:In this paper, I attempt to address the problem of how to model the survival curve in the early lifespan years; the neonatal years. I present a general model for a two group population in which the first group represents early stage failure and the second group represents the classical Gompertzian failure. After calcu...

journal_title：Mechanisms of ageing and development

authors： Witten M

更新日期：1986-01-01 00:00:00

abstract：:Activity of (Na+,K+)ATP-ase measured by means of influx of 86Rb was compared in lymphocytes from young, middle-age and old people. It was found that the (sensitive to ouabain) activity of (Na+,K+)ATPase was significantly diminished in the lymphocytes from aged subjects. ...

journal_title：Mechanisms of ageing and development

authors： Witkowski JM,Mysliwski A,Mysliwska J

更新日期：1985-12-01 00:00:00

abstract：:The activity of rat heart succinate dehydrogenase (SDH) increases 1.7-fold in old animals (Vitorica, J., Cano, J., Satrústegui, J. and Machado, A., Mech. Ageing Dev., 16 (1981) 105-116). This increase is due to an increase in enzyme protein itself because: (a) the activation state of the enzyme does not vary with age;...

journal_title：Mechanisms of ageing and development

authors： Velez M,Machado A,Satrústegui J

更新日期：1985-11-01 00:00:00

abstract：:Food intake, body weight and serum LH, FSH, progesterone and oestradiol-17 beta were monitored from weaning to puberty in fully fed females housed in groups of four or individually and in females individually housed and dietary restricted. Restriction of food intake from weaning delayed the onset of puberty (34-39 day...

journal_title：Mechanisms of ageing and development

authors： Holehan AM,Merry BJ

更新日期：1985-11-01 00:00:00

abstract：:The synthesis of sulfated proteoglycans in late fetal (19th to 22nd day of intrauterine life), early postnatal, and adult liver tissue as well as in hepatocytes and their distribution in plasma membranes were studied. Overall proteoglycan production is enhanced two-fold in fetal as compared with adult liver tissue. In...

journal_title：Mechanisms of ageing and development

authors： Gressner AM,Vasel A

更新日期：1985-09-01 00:00:00

abstract：:Age related change of the human minor pectoral muscles was biochemically demonstrated. Myosin-ATPase activity was significantly decreased with age, and was activated with Tris. The degree of the activation by Tris was observed to be lower in old aged patients than in the young. Furthermore, at low concentration of CaC...

journal_title：Mechanisms of ageing and development

authors： Nakano M,Baba H,Tauchi H,Sato T

更新日期：1985-07-01 00:00:00

abstract：:We have examined newly synthesized proteins in the young adult and in older populations of the nematode Caenorhabditis elegans using two-dimensional polyacrylamide gel electrophoresis (2D PAGE). A temperature-sensitive mutant strain, DH26, with a mean life span of about 15 days, under our conditions, was used to block...

journal_title：Mechanisms of ageing and development

authors： Johnson TE,McCaffrey G

更新日期：1985-05-31 00:00:00

abstract：:Whether lymphoid cells or their environment are responsible for the decreased tolerogen sensitivity of aged mice was examined using an adoptive transfer system. Bone marrow cells from old (24 months), middle aged (12 months) and young adult mice (2 months) were equally sensitive to tolerance induction with deaggregate...

journal_title：Mechanisms of ageing and development

authors： Habicht GS

更新日期：1985-04-01 00:00:00

abstract：:The DNA repair capabilities of cultured hepatocytes derived from five mammalian species in response to a broad range of ultraviolet light exposures were measured. Differences in the induction of DNA repair synthesis and the proportion of responding cells were noted only for the lowest fluences. These differences appea...

journal_title：Mechanisms of ageing and development

authors： Maslansky CJ,Williams GM

更新日期：1985-02-01 00:00:00

abstract：:Glycosaminoglycan (GAG) distribution has been analyzed in the adhesion sites, left substratum-bound after EGTA-mediated detachment, of various human skin fibroblast populations grown in vitro in the presence (asc+) or absence (asc-) of ascorbate. Examination of these skin fibroblasts during the EGTA treatment by scann...

journal_title：Mechanisms of ageing and development

authors： Beyth RJ,Culp LA

更新日期：1985-02-01 00:00:00

abstract：:Extrachromosomal circular DNA complexes from peripheral blood lymphocytes from inbred mice of accelerated senescence, were detected by mica-press-adsorption method. In senescence-resistant series (SAM-R), polydisperse circular DNAs were observed at 7 weeks of age and a restricted size class of small circular forms of ...

journal_title：Mechanisms of ageing and development

authors： Yamagishi H,Kunisada T,Takeda T

更新日期：1985-01-01 00:00:00

abstract：:The age-related studies of chromatin and DNA has attracted significant interest in recent years. However, individual works describe only some and a few of the many changes of chromatin. It is often difficult to decide whether these changes have secondary or primary nature. The overview of these studies makes it possib...

journal_title：Mechanisms of ageing and development

authors： Medvedev ZA

更新日期：1984-12-01 00:00:00

abstract：:The lipid peroxidation and activities of main enzymes involved in the peroxide metabolism were measured at resting conditions and after completion of a 2-month treatment with H2O2. At the start of the experiments 1- and 3-month-old female BALB/c mice received either a high dose of H2O2 (0.5% H2O2 in drinking water; in...

journal_title：Mechanisms of ageing and development

authors： Imre S,Tóth F,Fachet J

更新日期：1984-12-01 00:00:00